Figure 2 | Scientific Reports

Figure 2

From: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples

Figure 2

(A) Sensitivity of near full-length EV genome PCR tested on VIGR clinical stool samples (n = 65) and reference strains (n = 20) with EV infection detectable within 40 cycles by qPCR. PCR amplification was significantly affected by Ct value, with successful amplification resulting from Ct values ranging between 15.7 and 39.3. (B) Efficiency of near full-length EV genome PCR tested on VIGR clinical stool samples (n = 68) compared to sample age (years from collection to processing), with sequenced (red) and unsequenced samples (black). Mean sample age indicated. Sample age did not significantly affect outcome of amplification, with 6 samples over 10 years old producing a 7–8 kb EV amplicon using gel electrophoresis.

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