Figure 1
From: Tight Junction barriers in human hair follicles – role of claudin-1
Localization of Cldn-1 and TJ barrier in human anagen HFs. Immunohistochemical stainings of Biotin-SH (red; all pictures) and Cldn-1 (green; b,b′,d,d′,f,f′,h,and j in an overlay with Biotin-SH) in epidermis and infundibulum (a,b,b′), isthmus region (c,d,d′), central/suprabulbar region (e,f,g,g′, g′′,h) and in bulbar region (i,j) of anagen HFs. (b′,d′ and f′) represent magnifications of (b,d and f) demonstrating localization of the tracer stop in the SG of the infundibulum and CL of isthmus and central region (arrows indicate exemplarily one stop in each picture). (g′ and g′′) represent magnifications of the boxed areas in g (g′: upper box; g′′: lower box), and are overlay images of Biotin-SH (red) and DAPI (white) (left pictures) or Bitoin-SH alone (right pictures) showing tracer stop at Huxley’s layer and at the CL of the ORS (arrows) above keratogenous zone at Adamson’s fringe with differentiated Henle’s layer cells (g′), whereas below no tracer stop was found (g′′). (CL: companion cell layer; He: Henle’s layer; He*: differentiated Henle’s layer; Hu: Huxley’s layer). Scale bars: 20 µm.
