Figure 3
From: Tight Junction barriers in human hair follicles – role of claudin-1
Localization of TJ proteins and TJ barrier as well as differentiation markers in cultured anagen HF keratinocytes. Immunohistochemical stainings of TJ proteins Cldn-1 (a,a′,f), Cldn-4 (b,b′), Ocln (c,c′,g), ZO-1 (d,d′) (green) and Biotin-SH (e,e′,f,g) (red) and differentiation marker proteins Keratin 14 (K14) (h,h′), Filaggrin (Flg) (i,i′), and Keratin 75 (K75) (j,j′) (green) in ORS keratinocytes isolated from human HFs and cultured submerged on Transwell filters for 8 days. (k,k′) K75 in ORS keratinocytes cultured under air-liquid-interface conditions at day 8. (a′,b′,c′,d′,e′,h′,i′,j′,k′) Overlay images with DAPI (nuclei; blue) and phase contrast pictures (grey). (f,g) Overlay images of Biotin-SH (red) and Cldn-1 (f) and Ocln (g arrows denote Biotin-SH stops at Ocln) (green) respectively. Scale bars: 20 µm. Note the HF-like localization of TJ proteins and differentiation markers in the submerged cultures and the absence of HF-specific K75 in air-liquid interface-cultures.
