Figure 4
From: Tight Junction barriers in human hair follicles – role of claudin-1
Barrier function of cultured HF keratinocytes. (a) Representative time dependent TER curve of keratinocyte cultures isolated from ORS, SSK, and forehead/cheek under submerged culture conditions. Means ± SD; triplicate wells n = 3 different donors; significant differences are indicated with * (between ORS and forehead/cheek) and § (between ORS and SSK) and $ (SSK and forehead/cheek), respectively. (b) Time dependent TER curve of ORS keratinocytes isolated from infundibulum (black circles), and mid segment (open circles) of HFs (means ± SEM; triplicate wells n = 3 different donors). (c) Representative TER diagram (column plot) in an overlay with the corresponding permeation coefficients for FD4 (line plot) of ORS and SSK keratinocytes at different calcium concentrations at day 8. Means ± SD for line plot or + SD for columns; triplicate wells, n = 4 different donors; significant differences are indicated by § for TER and by * for FD4 permeation. (d) Representative time dependent TER curve of ORS and SSK keratinocytes cultured in presence or absence of fibroblasts (means ± SD; triplicate wells, n = 3 different donors; significant differences between keratinocytes grown in presence and absence of fibroblasts are indicated by § for SSK and by * for ORS. Statistical test for all: ANOVA.
