Figure 6

Mll1 deficiency alters neurogenic vs gliogenic capacity of late progenitor cells. (A) Schematic diagram showing experimental strategy for labeling late-born cells: P3 mouse pups were IP injected with EdU. At P14 retinas were collected to count numbers of four late-born cell types. (B,C) Distribution of EdU positive cells (red) in DAPI-labeled CreNeg (B) and Mll1KO (C) P14 retina cross-sections. Inserts (B’,B”,C’,C”) show details at higher magnification of the areas indicated. (D) Quantification of EdU positive cells across entire retinal cross-sections, three sections per sample and three samples per genotype. Results are represented as mean + SEM. Two-way ANOVA, **p < 0.0001. (E) Retina sections labeled for EdU (red) were co-stained with antibodies for cell fate markers (green) as indicated. (F) Cell counts for the different cell types derived from P3 EdU-labeled progenitor cells in Mll1KO retinas (red bars), normalized to counts from CreNeg littermates. Error bars represent SEM from three biological replicates (n = 3). *p < 0.05, n.s. = not significant by two-way ANOVA with Sidak’s multiple comparisons.