Figure 9
From: MLL1 is essential for retinal neurogenesis and horizontal inner neuron integrity
Mll1KO retinas have synaptic defects. Immunostaining of 1-month retina cross-sections for synaptic marker VGLUT1 (green) and bipolar marker PKC-A (red) (A,B), or for CtBP2 (Ribeye, green) and peanut agglutinin (PNA, red) for cones (C,D) reveal markedly reduced photoreceptor presynaptic terminals in the Mll1KO outer plexiform layer (OPL). Antibody information is given in Supplementary Table S2. In electron microscopy images of the OPL (E,F), representative rod spherules are marked with dotted lines and synaptic ribbons indicated by arrowheads. Quantification of the total number of rod spherules (G) and the number of spherules with normal ribbons (H), displayed as mean rod spherules per 10μm region + SEM. Quantification of the number of cone pedicles (I), displayed as mean cone pedicles per 10μm + SEM. *p < 0.05, n.s. = not significant by two-way ANOVA (n ≥ 4).
