Figure 6
Nox4 mediated anti-apoptotic role of argirein. (a) Representative western blot gel documents and summarized data showing the roles of argirein (AR, 1 μM) on Nox4 protein expression. After Nox4 siRNA transfection, Nox4 protein expression (b) and O2−. production with DHE staining (c) and the apoptotic-related protein expression including BAX, Bcl-2, and Caspase-3 (d–g) in RAECs incubated with high glucose (HG, 30 mM). Representative western blot gel documents and summarized data showing the roles of AR on ETAR protein expression (h,i) and the roles of specific ETAR inhibitor BQ123 on Nox4 protein expression (h,j) in RAECs. The original gel documents of western blot were shown in Fig. 6a,b,d,h in Supplementary Information. *P < 0.05 vs. Vehl or Scramble (Scr) Ctrl; #P < 0.05 vs. HG alone treated group (n = 4).
