Figure 4

L2A5 isotype determination using capture ELISA. 1:1000 dilution of L2A5 mAb was used in a capture ELISA with IgG1, IgG2a, IgGb, IgG3, IgM and IgA. Results are expressed as mean of absorbance (Abs) at 450 nm + standard deviation. Antibodies with known isotype B72.3 (IgG1) and CD15S (IgM) were used as controls (CTR).