Figure 6 | Scientific Reports

Figure 6

From: Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2–6 sialic acids

Figure 6

Western blot analysis of anti-STn mAbs binding to STn-expressing cell lines and MUC1 STn – IgG. MDA-MB-231 STn+ (A) and STn− (B) membrane extracts, as well as the chimeric protein MUC1 STn-IgG (C) were stained with L2A5 and 3F1 mAbs. Blotting was performed using non-treated membrane extracts (NT) and chimeric protein (MUC-Ig) as well as membrane extracts samples that were desialylated using sialidase (T) or unglycosylated MUC1 STn-IgG protein (Ung MUC-Ig). Representative western blots (cropped) are shown. Full length western blots are provided in Supplementary Fig. S2.

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