Figure 7
Immunohistochemical evidence of L2A5 mAb higher sensitive staining in bladder cancer samples. (A) Immunohistochemistry staining for L2A5 mAb in bladder cancer tissues. Sequential paraffin embedded tissue sections were stained with L2A5 and B72.3 mAb demonstrating the different staining patterns obtained. Higher extension and stronger signal intensity were observed using L2A5 mAb. (B) Immunohistochemistry of low-grade bladder cancer tissues. Sequential paraffin embedded tissue sections were stained with L2A5 and B72.3 mAb. Representative images show the increased sensitivity of L2A5 mAb (arrows) compared to lack of staining using B72.3. (C) Immunohistochemistry of metastatic bladder cancer tissue using L2A5 mAb. Staining of paraffin embedded tissue shows specific tumour cell staining (arrows) and absence of staining in lymphocytic cells, vessels and connective tissue. (D) Sialic acid-dependence of L2A5 staining. Immunohistochemistry staining using L2A5 was performed in non-treated and sialidase treated paraffin embedded bladder cancer tissue. Representative images show complete absence of staining after sialidase treatment.
