Table 1 Expression, capture, and light release efficiency of hCNTF with immobilization on the surface of magnetic beads.

From: Accelerated pharmaceutical protein development with integrated cell free expression, purification, and bioconjugation

CFPS system

Total amount hCNTF [mg/L]a

Amount hCNTF Captured beads [%]

Amount hCNTF Releasedb [%]

BYLc

20.0

84

>6.3e

HeLac

49.2

93

ND

HeLad

ND

ND

>19e

  1. aMilligram hCNTF per liter of CFPS matrix determined with hCNTF standard in the WBs; bThe percentages are based on the relative volumes of the bands of hCNTF released from the magnetic beads compared to the total synthesized hCNTF in the same WB gels; cImmobilized peptide followed by split-intein mediated capture, washing, and light triggered release; dsplit-intein mediated capture and protein trans splicing in solution followed by immobilization, washing, and light triggered release; ecorrected values are based on negative controls (Fig. S4). ND = Not Determined.
Back to article page