Table 3 Photocleavable peptides used in this study.
Peptide number | NpuDnaEC16 peptide sequence with non-canonical amino acid/ conjugation in bold | Non-canonical amino acid/conjugation | Peptide sequence introduced on C-terminus of POI via PTS |
|---|---|---|---|
1 | DGHNFALKNGFIASNCFGSKHHHHHH | N/A | CFGSKHHHHHH |
2 | DGHNFALKNGFIASNCF(2-NO2)GSKHHHHHH | F(2-NO2): 2-Nitrophenylalanine22 | CF(2-NO2)GSKHHHHHH |
3 | DGHNFALKNGFIASNC-ANP-GSKHHHHHH | ANP: 3-(Amino)-3-(2-nitrophenyl)propionic acid20 | C-ANP-GSKHHHHHH |
4 | DGHNFALKNGFIASNCF-X-GSKHHHHHH | X: Photolinker CAS 162827-98-721 | CF-X-GSKHHHHHH |
5 | DGHNFALKNGFIASNCGSG-X-GGHHHHHH | X: Biotin on side chain of Lysine | CGSG-X-GGHHHHHH |
6 | DGHNFALKNGFIASNCGSG-XY-GGHHHHHH | X: Biotin on side chain of Lysine Y: Photolinker CAS 162827-98-721 | CGSG-XY-GGHHHHHH |
7 | DGHNFALKNGFIASNCGSG-XY-GGEPEA | X: Biotin on side chain of Lysine Y: Photolinker CAS 162827-98-7 | CGSG-XY-GGEPEA |
