Figure 4
From: Bundles of Brain Microtubules Generate Electrical Oscillations
Electrical oscillations and coupling in membrane-permeabilized neurites. (a,b) Images of one-week old cultured hippocampal neurons(x20), before and after cell membrane permeabilization by addition of 1% (v/v) Triton X to the bathing solution. (c,d) Permeabilized neurites before and double patching with patch-clamp pipettes as shown in the actual locations of electrical recording. (e) Spontaneous electrical oscillations observed in one pipette and mirror images collected on the second pipette. Time series were obtained for a ±1 mV bias applied to one of the pipettes, as shown on the Top and bottom tracings, respectively. (f) Power spectra of four times the length of the tracings shown on (e), for the positive (Black) and negative (Gray) biased signals, respectively. Linear-Linear plots for respective spectra are also shown (Bottom). Raw data were Gaussian-filtered at 50 Hz for display purposes only. Data are representative of three independent experiments.
