Figure 5

TrkB and pTrkB-T816 expression 24 h post-ischemia. (A) Representative Western blots showing TrkB and pTrkB expression at P7. The blots presented for each group were cropped from separate gels for improvement in clarity and conciseness of presentation. Vertical solid black lines delineate the separate blots between groups, while horizontal dotted black lines delineate separate proteins run on different gels for the same sample. All gels were run in the same experimental conditions (see methods for details). (Full-length blots presented in Supplementary Figure 5). (B) Quantification of blots shown in A. TrkB is upregulated by ischemia and downregulated by each dose of ANA12 + PB. (C) Percent contralateral expression of TrkB and pTrkB. TrkB percent contralateral is unchanged; pTrkB percent contralateral is downregulated by ischemia and rescued by 5 mg/kg of ANA12 + PB. (D) TrkB to pTrkB ratio. pTrkB ratio is upregulated by ischemia and rescued in a dose-dependent manner by ANA12 + PB. (E) Western blots showing TrkB and pTrkB expression at P10. (F) Quantification of blots shown in E. TrkB and pTrkB are unaffected by ischemia at P10. (G) TrkB and pTrkB percent contralateral. Both TrkB and pTrkB were unchanged by ischemia. (H) TrkB to pTrkB ratio. Significance to PB alone was done using One-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001. Significance to naïve control: ^#P < 0.05, ^##P < 0.01, ^###P < 0.001. Significance to ANA12 5 + PB: ^@P < 0.05, ^@@P < 0.01, ^@@@P < 0.001. Ipsilateral versus contralateral: ^γP < 0.05, ^γγP < 0.01, ^γγγP < 0.001.