Figure 7

vGLUT1 vs. vGAT expression 24 h post-ischemia. (A) Representative Western blots showing vGLUT1 and vGAT expression at P7. The blots presented for each group were cropped from separate gels for improvement in clarity and conciseness of presentation. Vertical solid black lines delineate the separate blots between groups, while horizontal dotted black lines delineate separate proteins run on different gels for the same sample. All gels were run in the same experimental conditions (see methods for details). (Full-length blots of each tested presented in Supplementary Figure 7). (B) Quantification of Western blots shown in A. vGLUT1 is downregulated by ischemia and rescued by all doses of ANA12 + PB in a dose-dependent manner. vGAT is not modulated by ischemia. (C) Representative Western blots showing vGLUT1 and vGAT expression at P10. (D) Quantification of blots shown in C. vGLUT1 is downregulated by ischemia and rescued in a dose-dependent manner by ANA12 + PB. vGAT is unaffected by ischemia. Significance to PB alone: *P < 0.05, **P < 0.01, ***P < 0.001. Significance to naïve control: ^#P < 0.05, ^##P < 0.01, ^###P < 0.001. Significance to 5 mg/kg of ANA12 + PB: ^@P < 0.05, ^@@P < 0.01, ^@@@P < 0.001. Ipsilateral to contralateral: ^γP < 0.05, ^γγP < 0.01, ^γγγP < 0.001.