Figure 4

Ndr1 and Ndr2 deletion promote retinal cell death. (A) Retinas from P28 WT, Ndr2 and Ndr1 KO mice were labeled with TUNEL (green). Nuclei labeled with Hoechst 33342 (blue). Insets (dashed boxes) are shown in Suppl. Fig. S2A. (B) Active caspase-3 (red) and Pax6 (green) immunofluorescence in WT, Ndr2 and Ndr1 KO retinas. Arrows show representative caspase 3-positive and Pax6-positive cells. Scale bars for (A and B) 20 μm. (C) Active caspase-3 (red) and syntaxin (green) in WT, Ndr2 and Ndr1 KO retinas. Images in C were acquired by confocal microscopy and visualized as single optical sections. Scale bar, 5 μm. (D) The number of TUNEL-positive nuclei were counted manually in 500 μm long region of interest (ROI) and plotted; n ≥ 3 mice per genotype. (E) The number of active caspase-3 positive nuclei were counted manually in 500 μm ROIs and plotted; n ≥ 2 mice per genotype. Error bars represent SD and asterisks indicate levels of significance using an unequal variance t-test (p < 0.05).