Figure 6
From: Ndr kinases regulate retinal interneuron proliferation and homeostasis
Ndr deletion disrupts amacrine cell homeostasis in adult mouse retina. (A) Immunofluorescence microscopy of the mitotic marker pHH3 (green) and calretinin (red) in P28 WT and Ndr KO mouse retinas. Arrows point to representative pHH3 and calretinin-positive nuclei. Scale bar, 20 μm. (B) Confocal immunofluorescence microscopy of pHH3 (red) and the pan-amacrine protein syntaxin 1 (green) in the basal INL of P28 WT and Ndr KO mice. Arrows point to pHH3-positive cell with perinuclear syntaxin 1. Images were acquired by confocal microscopy and visualized as merged z-sections. Nuclei labeled with Hoechst 33342 (blue). Scale bar, 5 μM. (C) pHH3 (red) and HuD (green) immunofluorescence. (D) GAD65 (red) immunofluorescence. Scale bar, 40 μm. (E) The percentage of Pax6-positive, calretinin-positive syntaxin positive and HuD-positive mitotic (pHH3) cells from 500 μm long ROIs were quantified and plotted. (F) Relative GAD65 immunofluorescence within the INL of Ndr KO retinas was quantified and plotted as ratios to WT by counting the number of pixels within five 100 μm long ROIs per retinal section (n = 3 mice per genotype). SD and statistical significance were determined by unequal variance t-test (p < 0.05).
