Figure 8
Wax-embedded tissue imaged by SRμCT is suitable for subsequent histology and the resulting images can be aligned to tomograms. (A,A’) NeuN immunostaining of 7 μm thick transverse tissue sections demonstrate that immunolabelling of tomographically-imaged tissue is also possible. (B,B’) A ‘6.4 μm’ tomographic slice (mean z-projection of 4 optical slices to match mechanically sectioned, 7 μm slice) matched to the NeuN–stained image in (A) with consistent morphological features including vasculature (red arrows) and the central canal (yellow arrow). At low magnification, dorsal roots are also seen to align (black arrowheads in A, B). At higher magnification, some motoneuron cell bodies (cyan arrows) can be identified in tomograms. (C,C’) Haematoxylin and eosin staining of 7 μm thick transverse tissue sections reveals that histological staining of tomography tissue is also possible. (D,D’) A ‘6.4 μm’ slice from a matched tomogram, again showing consistent features including vasculature (red arrows), the central canal (yellow arrows) and large motoneurons (cyan arrows).
