Figure 1 | Scientific Reports

Figure 1

From: Biochemical analysis of GTPase FlhF which controls the number and position of flagellar formation in marine Vibrio

Figure 1

Characterization of FlhF protein. (A) Cell motility. Cells of the flhF mutant (LPN1) harboring a plasmid, vector; pBAD33, FlhF; pAK322, or FlhF-His6; pTSK119, were inoculated into a soft agar plate containing 0.02% arabinose and incubated for 6 hours at 30 °C. (B) Cells of BL21(DE3) harboring a plasmid pTSK110 which has flhF-his6 in pColdIV vector were broken in the presence and absence of MgCl2 or GTP. The proteins were separated by SDS-PAGE and the gels were stained by CBB. The regions of interest were cropped from the gels. 1: the pre-induction cells, 2: the post-induction cells, 3: the low speed centrifugal precipitation, 4: the low speed supernatant.

Back to article page