Table 1 Bacterial strains and plasmids used in this study.
Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
V. alginolyticus | ||
VIO5 | VIK4 (Rifr Pof+ Laf−) | |
LPN1 | VIO5 ΔflhF (Rifr Pof+ Laf−) | |
E. coli | ||
DH5α | Recipient for DNA manipulation |  |
BL21(DE3) | Â | Novagen |
BL21(DE3)/pLysS | Â | Â |
Plasmids | ||
pBAD33 | Cmr, PBAD | |
pTY57 | Cmr, PBAD, MCS from pBAD24 | |
pCold IV | Ampr, PcspA | Takara |
pAK322 | flhF (wt) in pBAD33 | |
pAK325 | flhF-egfp (wt) in pBAD33 | |
pTSK110 | flhF-his6 in pCold IV | This study |
pTSK119 | flhF-his6 in pTY57 | This study |
pSK102 | flhF (E440K) in pBAD33 | |
pSK111 | flhF (R334A) in pBAD33 | This study |
pSK202 | flhF-egfp (E440K) in pBAD33 | |
pSK211 | flhF-egfp (R334A) in pBAD33 | This study |
pSK301 | flhF-his6(G299A) in pCold IV | This study |
pSK302 | flhF-his6(G304A) in pCold IV | This study |
pSK303 | flhF-his6(K305A) in pCold IV | This study |
pSK304 | flhF-his6(T306A) in pCold IV | This study |
pSK305 | flhF-his6(K311A) in pCold IV | This study |
pSK306 | flhF-his6(D377A) in pCold IV | This study |
pSK307 | flhF-his6(G380A) in pCold IV | This study |
pSK308 | flhF-his6(T436M) in pCold IV | This study |
pSK309 | flhF-his6(K437A) in pCold IV | This study |
pSK310 | flhF-his6(D439A) in pCold IV | This study |
pSK311 | flhF-his6(E440K) in pCold IV | This study |
pSK312 | flhF-his6(D470A) in pCold IV | This study |
pSK313 | flhF-his6(R334A) in pCold IV | This study |
pTrc-flhG | his6-tev-flhG in pTrcHisB | |
