Figure 2

Immunoblot assay of Leishmania membrane proteins, LAg with (A) 9 infected urine samples (VL1-VL9) and (B) 8 non-VL urine samples including non-endemic healthy controls (C1&C2), endemic healthy controls (C3&C4), malaria (M1), viral fever (V1), tuberculosis (TB1) and typhoid (T1) with an infected VL urine sample (VL). Molecular weight marker lane, M, is marked in the left in kilodaltons. Corresponding blots in part A have been cut, treated and arranged from a single gel. Similarly blots in part B belong to a single gel. Images of reactive Leishmania membrane proteins to infected (C) and healthy (D) urine samples were obtained through Image Lab software and urine reactive proteins were marked as peaks in kDa.