Figure 5
Klebsiella oxytoca hampers gut barrier function in cachectic mice. (A) Fecal K. oxytoca levels in healthy mice receiving the vehicle (CT), in C26-transplanted mice receiving the vehicle (C26) and in C26-transplanted mice receiving K. oxytoca in their drinking bottle (C26-Kox). (B) In black, number of mice for which coliform bacteria were detected in their mesenteric lymph nodes (MLN). (C) Fecal K. oxytoca levels in mice displaying coliform bacteria in their MLN and in mice free of coliform bacteria in their MLN, within the C26-Kox group. (D–G) Ileal mRNA expression levels of key markers involved in gut permeability, epithelium renewal, mucosal immunity and antimicrobial peptide production. Data are presented as whiskers plots with minimal and maximal values (A) or mean ± SEM (C–G). The dotted line indicates the expression level in sham-injected mice. N = 7–8. #p < 0.1; *p < 0.05; **p < 0.01; ***p < 0.001.
