Figure 2
From: DAF-21/Hsp90 is required for C. elegans longevity by ensuring DAF-16/FOXO isoform A function
DAF-21/Hsp90 is required for normal lifespan and affects reduced ILS conferred longevity. daf-21(RNAi) employed either from hatching (a) or from the L4 stage (b) reduces wild-type lifespan to the level of the daf-16(mgDf50) mutant (p < 0.001 and p < 0.001 respectively). The daf-2(e1370) allele significantly increases the lifespan of worms with wild-type daf-16 (p < 0.001) but not that of daf-16(mgDf50) mutants (p = 0.958). daf-21(RNAi) employed from hatching (c) reduces, whereas from the L4 stage (d) does not significantly affect the extended lifespan of daf-2(e1370) mutants (p = 0.001 vs. p = 0.076). Likewise, daf-21(RNAi) treatment from hatching reduces, but from the L4 stage does not affect the lifespan of daf-2(e1370); daf-16(mgDf50) double mutants (p < 0.001 and p = 0.435 in panel c and d, respectively). Lifespan assays were repeated three times. Survival curves were compared using the Kaplan-Meyer log rank test. Lifespan values are given in Supplementary Table S1. EV: empty vector RNAi.
