Figure 2

(A) Flow cytometric analysis of EGFP-Bax, -Bak or Bok expressing HCT116/WT and HCT116/DKO cells shows apoptosis associated exposure of phosphatidylserine by Annexin-V-APC staining. (B) Expression of EGFP-Bax, -Bak or –Bok is associated with decrease of the mitochondrial membrane potential in the presence (WT) and absence (DKO) of Bax&Bak shown by TMRE (TMRE^low). (C) Number of Annexin-V-APC positive apoptotic cells is reduced by co-expression of mCherry-Mcl-1 as compared to EGFP-Bax, -Bak or –Bok expression alone in the presence (WT) and absence (DKO) of Bax and Bak. (D) Number of EGFP-Bax, -Bak or -Bok expressing cells showing dissipation of the mitochondrial membrane potential (TMRE^low) is reduced by co-expression of mCherry-Mcl-1 independent of Bax&Bak.