Figure 1
From: Strong increase in the autofluorescence of cells signals struggle for survival
Effect of ampicillin treatment on the forward light scattering and autofluorescence intensity of E. coli cells. E. coli 7705035 [MIC = 4 mg/L; (a) and (b)] and 8812112 [MIC = 64 mg/L; (c) and (d)] cells were treated with a range of ampicillin concentrations for 3 hours of the exponential growth. Autofluorescence and forward light scattering signal (FSC) of treated cells and non-treated control were measured after 1, 2 and 3 hours of incubation using the Gallios flow cytometer. (a) and (c) The change in the distribution profiles of the cellular autofluorescence (λex 488 nm/λem 525/20 nm) over time, for the ampicillin concentrations: 0 (green), 4 (red) and 64 mg/L (blue). (b) and (d) 2-dimensional representation of the FSC and FL1 (λex 488 nm/λem 525/20 nm) of each event (n = 50,000) at 1, 2 and 3 hours of treatment with 0, 4, and 64 mg/L of ampicillin. This figure presents results of one representative experiment which was independently repeated three times. The overlap of green and red produces the khaki area.
