Figure 5
From: Strong increase in the autofluorescence of cells signals struggle for survival
Effects of gentamicin and sodium hypochlorite treatment on the autofluorescence of E. coli cells. E. coli 7705035 cells were treated with a range of gentamicin concentrations corresponding to 0 (red), 0.125 (light blue), 0.25 (orange), 0.5 (MIC; light green), 1 (dark green), 2 (brown) and 4 mg/L (violet) or 0.08% sodium hypochlorite (grey) for 3 hours of the exponential growth. Autofluorescence and FSC of treated cells and untreated control were measured after 1, 2 and 3 hours of incubation using a flow cytometer. Cell autofluorescence was normalized by the FSC signal to obtain the autofluorescence per FSC unit. (a), (b) and (c) 1, 2 and 3 hours of gentamicin treatment respectively. The overlap of red, light blue, orange, light green and dark green produces the brown area. (d), (e) and (f) 1, 2 and 3 hours of sodium hypochlorite treatment respectively. This figure presents results of representative experiments, which were independently repeated 4 times.
