Table 1 Expression of 28 genes coding for flavoproteins, the soxR and lacZ genes in ampicillin-treated E. coli cells.

From: Strong increase in the autofluorescence of cells signals struggle for survival

 

Ampi 1/Ampi 0

Ampi 2 (MIC)/Ampi 0

Ampi 4/Ampi 0

Mean fold increase

Standard error

p value

Mean fold increase

Standard error

p-value

Mean fold increase

Standard error

p-value

Controls

lacZ

0.97

0.06

reference

1.19

0.08

reference

3.25

0.23

reference

lacZ IPTG

0.98

0.01

0.9694

1.23

0.03

0.9674

2.68

0.11

0.7101

Protection against ROS and reactive nitrogen species (RNS)

soxR

0.97

0.15

0.747

2.36

0.41

0.0062

11.29

1.46

0.0003

fpr

0.92

0.06

0.7568

2.14

0.13

0.0035

7.43

0.58

0.0158

nfnB

1

0.08

0.7189

2.29

0.31

0.0024

10.93

0.51

<0.0001

norV/norW

1.23

0.1

0.0908

3.77

1.04

0.0003

14.91

1.42

<0.0001

Genes coding for the respiratory chain components

poxB

0.86

0.22

0.4071

2.23

0.28

0.006

8.21

1.5

0.0096

ndh

1.02

0.06

0.4608

1.86

0.09

0.0368

10.66

0.78

0.0001

nuoA/nuoF

1.38

0.13

0.0192

3.29

0.64

0.0006

10.9

1.61

0.0006

ycaK

1.26

0.08

0.0221

2.41

0.35

0.0044

8.9

0.36

0.0029

Genes coding for proteins involved in different metabolic pathways

cysJ

1.92

0.73

0.0499

3.78

0.19

<0.0001

17.01

2.42

<0.0001

ssuD/ssuE

1.15

0.27

0.0704

5.96

1.18

<0.0001

16.33

2.01

<0.0001

aroC

1.14

0.3

0.2625

2.91

0.39

0.0004

19.1

2.43

<0.0001

ilvI

1.59

0.18

0.006

3.04

0.47

0.0003

11.07

0.94

0.0002

metF

0.77

0.05

0.1393

1.89

0.33

0.0443

7.02

1.12

0.0347

dfp

1

0.08

0.768

2.1

0.29

0.0088

9.55

0.58

0.0007

mqo

1.23

0.1

0.0699

3.15

0.38

<0.0001

9.76

1.95

0.0024

wrbA-1

1.11

0.04

0.0998

1.99

0.07

0.01

4.96

0.66

0.3019

wrbA-2

1.1

0.03

0.1287

1.78

0.06

0.0689

4.37

0.28

0.4863

wrbA-3

1.04

0.03

0.3303

2.3

0.09

0.0009

5.17

0.7

0.2311

frdA

1.18

0.15

0.2568

2.13

0.46

0.0062

6.12

0.86

0.1119

ycdH/ycdM

1.04

0.08

0.5753

2.78

0.42

0.0005

9.43

1.04

0.0017

Not induced

fldA

1.01

0.02

0.6475

1.64

0.07

0.1935

7.74

0.39

0.0086

gcl

0.79

0.04

0.1099

0.77

0.08

0.5638

7.31

0.75

0.018

glpA

1.07

0.01

0.2036

1.59

0.04

0.2704

5.02

0.34

0.2777

glpD

1

0.01

0.7167

1.76

0.09

0.0802

4.19

0.24

0.5631

lpd

0.95

0.05

0.8387

1.61

0.06

0.2186

6.3

0.11

0.0683

pdxH

1.01

0.03

0.6753

1.5

0.04

0.4295

5.26

0.22

0.2582

sdhA/sdhC

0.85

0.03

0.2015

1.31

0.07

0.7818

3.4

0.32

0.9274

uxaC

1.08

0.05

0.2231

1.7

0.07

0.1295

6.34

0.35

0.072

yieF/yieE

0.76

0.03

0.0772

0.96

0.08

0.7004

2.81

0.29

0.777

  1. Promoters of the tested genes were fused to the gene coding for the fast-folding green fluorescent protein (GFP; λex 488/9/λem 525/20 nm). E. coli MG1655 strains (MIC ampicillin = 2 mg/L) carrying plasmids with these transcription reporter fusions were grown with a range of ampicillin concentrations. After 3 hours of incubation, GFP fluorescence (λex 488/9/λem 525/20 nm) and OD600nm of treated cells and non-treated control were measured using a fluorimeter. Fluorescence was normalized by the OD600nm. Each value represents the mean (+/−standard error) of the fluorescence increase compared with the non-treated condition of each strain from six independent experiments. Significant differences with the lacZ control according to the 1-way ANOVA Dunn’s test < 0.05 are presented in bold.
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