Figure 3
Effects of DPAmix on neutral lipid degradation in CHO-K1 cells. (a,b) CHO-K1 cells were incubated for 24 h with [14C]oleic acid to accumulate [14C]lipids. After free [14C]oleic acid was removed, and cells were treated with DPAmix for 0–12 h. (a) At indicated time, the cells treated with DPAmix 0 (♦), 0.12 (▲), 1.2 (●) and 12 μM (■) were lysed and cellular [14C]TG (left) and [14C]CE (right) were detected by image analyzer. The results obtained were plotted as % of control (without drugs). Values represent means ± SD (n = 3 ~ 4). (b) At indicated time, the cells treated with DPAmix 12 μM and the medium fractions were recovered to analyze the [14C]lipids in each fraction using image analyzer. The total amount of [14C]lipids in medium and cells is taken as 100% at each time. (c) CHO-K1 cells were cultured with 20 μM oleic acid. After the 12 h incubation, the lipid droplet-containing CHO-K1 cells (0 h) were incubated with DPAmix for 6 h, and were stained with oil red O. Bars; 20 μm.
