Figure 4

DOCK8 interacted with ILK in equine PBL. Representative western blots of immunoprecipitations of DOCK8 (A) and ILK (B). ILK was detected at 59 kDA in input lysates of PBL (input PBL) as well as after immunoprecipitation of DOCK8 (A). DOCK8 was detected at 190 kDA in input lysates of PBL (input PBL) as well as after immunoprecipitation of ILK (B), verifying an interaction between these two proteins. In respective isotype controls (rb IgG; mIgG) and blank (IP buffer), neither ILK nor DOCK8 was detected.