Figure 6
From: Hematoxylin and eosin staining of intact tissues via delipidation and ultrasound
Possible principle of iHE. A fixed tissue is used as the original tissue. After dehydration, the tissue is subjected to delipidation, followed by the H&E staining procedure; the H&E staining process was simplified so that the reader could better understand the principle for how ultrasound is involved in the process. Dichloromethane can cause a porous state of the cell membrane, equal to the porous state of the tissue. The pores of the tissue change in the ultrasonic field because ultrasound is a longitudinal wave. Thus, the pores in the wave crest of the ultrasound are stretched, while the pores in the trough of the waves are compressed. Meanwhile, the random motion of particles is enhanced in the ultrasonic field. The diffusion process is enhanced and achieves rapid and uniform staining.
