Table 1 NMN production in E. coli BL21(DE3)pLysS transformed cells.
From: β-nicotinamide mononucleotide (NMN) production in Escherichia coli
Vector | Growth medium | NAM (%) | Glucose (%) | Inductor | [NMN] mM in bacterial cells | [NMN] mg per gram of protein | [NMN] mg per L of culture | OD 600 |
|---|---|---|---|---|---|---|---|---|
pET28a-hdNadV | LB | 0.1 | 1 | IPTG | 23.57 ± 7.2 | 18.70 ± 5.73 | 10.89 ± 2.5 | 1.3–1.6 |
LB | 1 | 1 | IPTG | 20.79 ± 1.92 | 16.63 ± 1.52 | 14.33 ± 0.76 | 2.2–2.4 | |
pET28a-baPrs-hdNadV | LB | 0.1 | 1 | IPTG | 20.24 ± 1.84 | 16.06 ± 1.46 | 13.69 ± 1.17 | 2.1–2.2 |
LB | 0.1 | — | Lactose | 16.86 ± 3.27 | 13.37 ± 2.60 | 8.57 ± 1.56 | 1.6–1.8 | |
PYA8 | 0.1 | — | Lactose | 22.63 ± 2.14 | 17.26 ± 1.63 | 15.42 ± 1.47 | 2.3–2.6 | |
LB | 1 | 1 | IPTG | 22.89 ± 1.28 | 18.16 ± 1.01 | 11.06 ± 0.63 | 1.4–1.6 | |
pET28a-hdNadV and pET15b-PRS135 (co-transformed separate vectors) | LB | 0.1 | 1 | IPTG | 9.28 ± 0.36 | 7.36 ± 0.28 | 6.95 ± 0.54 | 1.3–2.2 |
