Figure 3 | Scientific Reports

Figure 3

From: Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae

Figure 3

Accumulation of lipid droplets and TAGs by CmGPAT1 overexpression. (a) BODIPY staining of GPc, GP1, and GP2 cells. Each cell was grown under normal growth conditions until OD750 = 0.4–0.6, and was stained with BODIPY. Bright field (top) and BODIPY staining (bottom) images are indicated. Each BODIPY staining (yellow-green signal) image was merged with the relevant chlorophyll fluorescence (red signal) image. Bar, 2 μm. (b) Intracellular TAG content in GPc, GP1, and GP2 cells. Values are averages of three independent experiments and represent percentages of dry weight. Error bars indicate standard deviation (SD). Asterisks indicate a significant difference compared with GPc (Student’s t-test, P < 0.05). (c) Fatty acid composition of the purified TAGs in GPc, GP1, and GP2 cells. Fatty acid components of the TAGs are indicated as a percentage of the fatty acid composition. Each fatty acid is indicated by the number of carbons:number of double bonds. Values are averages of three independent experiments. Error bars indicate SD. Asterisks indicate a significant difference compared with GPc (Student’s t-test, *P < 0.05; **P < 0.01).

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