Figure 2
Nan- and erythroid niche-specific expression changes. Expression of (a) Irf7 and (b) Hbb-bh1 is not differentially regulated in Nan vs. WT-PHB spleen erythroid precursor cells while both are markedly upregulated in Nan fetal liver (fold change in Nan fetal liver from Supplemental Table 2 in blue). (c) Expression of Bcl11a. Fold change vs. WT-PHB in blue. (d) Confirmation of Hbb-bh1 (top) and Bcl11a (bottom) expression by quantitative RT-PCR. AU, arbitrary units, no significant differences. n = 3 for Nan and WT-PHB. (e) Hbb-bh2 is progressively upregulated during erythropoiesis in Nan, reaching a 534-fold increase at the orthochromatophilic stage. (f) Confirmation of dramatically up-regulated Hbb-bh2 expression in Nan by quantitative RT-PCR. Average values for WT-PHB are shown in blue to facilitate comparison to Nan. AU, arbitrary units; *p < 0.01 and **< 0.001 Nan vs. WT-PHB. n = 5 (Nan) and 6 (WT-PHB) except Ortho (n = 2 and 3, respectively). (g) Adult β-globin, Hbb-bs, is downregulated in Nan pro- and basophilic erythroblasts. The duplicated adult globin locus, Hbb-bt, shows the same pattern (http://nanexpression.mdibl.org/). Neither Hbb-bh2 nor Hbb-bt are differentially expressed in Nan whole fetal liver. Tick marks, mean ± SD. Note: All expression values are log2-transformed, normalized counts; therefore, values below 1 are considered background (no expression).
