Figure 7
ROS generation, mitophagy, and DNA damage in Nan. (A) Quantitation of ROS production in Ter119 positive, CD71 low (mature red blood cells) and Ter119 positive CD71 high (reticulocytes) populations. As expected, DCF and DHE production in mature red cells is lower than in reticulocytes43. n = 3 per group. (B) Flow cytometry of MitoTracker Red (MTR) and Thiazole Orange (TO) stained WT and Nan red blood cells to assess mitochondria and ribosome content three days post-phlebotomy. Early reticulocytes are ribosome-positive, mitochondria-positive (upper right quadrant), late reticulocytes ribosome-positive, mitochondria-negative (lower right quadrant), and retained mitochondria ribosome-negative, mitochondria- positive (upper left quadrant). Cytograms representative of 3 samples/group. (C) Representative micronucleus assay results in non-irradiated mice. (D) Quantitation of induced micronuclei 48 and 72 hours following irradiation. n = 4 per group; ***p < 0.001 (within group comparison at 0 and 48 hours). PI, propidium iodide.
