Figure 2 | Scientific Reports

Figure 2

From: The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp

Figure 2

Linear gfp DNA uptake assay of strains expressing comE, crp, or comE-crp. For all plots side scatter (SSC) is plotted against GFP fluorescent intensity (GFP). (a) Naturally competent Bp 1026b and Bt E264 show 63–75% of cells expressing GFP after incubation with gfp. E. coli constitutively expressing gfp (GFP+) shows 96.8% of cells expressing GFP in contrast to wildtype E. coli (GFP-) showing no GFP expression. (b) Wildtype (WT) and attTn7 controls (Prha) of Bp K96243, Bm ATCC23344 and Bc K56-2 show no GFP expression indicating their inability to uptake gfp. However, expression of comE, crp or comE-crp empowered natural competency, showing 39–73% of cells expressing GFP.

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