Figure 4

In vitro attenuation of Bm Δasd strains in RAW264.7 murine macrophages. Bm strains ATCC23344 (a), Ivan (b), China 5 (c), 2002721278 (d) were able to replicate well within the intracellular environment (circles) while the Δasd counterparts showed complete abolishment of the ability to replicate intracellularly (squares). Complementation of the Δasd gene in each Bm strain rescued the intracellular replication defect (triangles).