Figure 5
In vivo attenuation of Bm Δasd strains in BALB/c intranasal challenge. BALB/c mice (n = 5) were challenged intranasally with 1 × 107 CFU of Bm strains ATCC23344 (a), Ivan (b), China 5 (c), 2002721278 (d) and their Δasd counterparts. Survival was monitored for 63 days (left panels). Surviving mice were sacrificed and bacterial burdens from the lungs, liver, and spleen were determined by serial dilution and plating (right panels).
