Figure 1 | Scientific Reports

Figure 1

From: In vivo imaging of bacterial colonization of the lower respiratory tract in a baboon model of Bordetella pertussis infection and transmission

Figure 1

Characteristics of the B. pertussis B1917-GFP-expressing strain. (A) Plasmid map of the GFP vector, pBBPG, used for transformation. Rep: Plasmid Replication origin, Mob: Mobility gene locus essential for conjugative DNA processing, cat: chloramphenicol acetyltransferase (chloramphenicol resistance gene), gfp: Green Fluorescent protein gene, Ppor: promotor of the major porin gene from B. pertussis. (B) GFP bacterial fluorescence observed by confocal microscopy. All bacteria are detected by transmission light (grey) and GFP-expressing bacteria (green) are visible in the focal plane. (C) Differential growth of B1917- WT (grey) and B1917-GFP-expressing (green) strains in liquid culture without antibiotics (Stainer-Scholte medium).

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