Figure 5 | Scientific Reports

Figure 5

From: In vivo imaging of bacterial colonization of the lower respiratory tract in a baboon model of Bordetella pertussis infection and transmission

Figure 5

In vivo imaging by pCLE coupled with bronchoscopy in B. pertussis B1917-GFP infected baboons. Images (A–F) extracted from pCLE acquisition in the trachea at D2 (A–C) and D14 (D–F) post-infection of one animal, representative of the three challenged baboons. B1917-GFP bacteria (aggregates and biofilms) are detected in 488-nm channel (green) (A,D) and APCs are labeled with anti-HLA-DR AF647 antibody and detected in the 660-nm channel (red) (B,E). (G) Image taken with the bronchoscope camera showing the pCLE probe (arrow) in the trachea. (H) Evaluation of B. pertussis B1917-GFP colonization in the trachea determined by the area of GFP signal in the 488-nm channel (n = 3 animals) and comparison of the data between timepoints (****p < 0.0001).

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