Figure 2

ATC application downregulated pluripotency markers in hESCs. (A) Bright field images of single ESCs and integrin-bound MBs (arrows). Confocal fluorescence images of single hESCs stained with DAPI (blue), Oct4 (magenta), Nanog (yellow), and Sox2 (green) with and without ATC application (30 min). Scale bars: 50 µm. (B) Percentages of hESCs with Oct4, Nanog, and Sox2 after ATC treatment compared with control. (C) Bright field images of a hESC colony and integrin-bound MBs (arrows). Confocal fluorescence images showing hESCs stained with DAPI (blue), Oct4 (magenta), Nanog (yellow), and Sox2 (green) with and without ATC application. Scale bars: 50 µm. (D) Corrected fluorescence intensity measurement of hESCs with Nanog, Oct4, and Sox2 after ATC treatment. (E) Immunoblot analysis of protein expression in hESCs. All quantifications were from at least 3 independent experiments with two replicates per experiment. Unpaired t test p values * < 0.05, **<0.01, and ***<0.001; n.s, not significant.