Figure 3
From: Acoustic Tweezing Cytometry Induces Rapid Initiation of Human Embryonic Stem Cell Differentiation

ATC application activated FAK signaling and induced differentiation of hESCs. (A) Adherent colony of hESCs stained with DAPI (blue), E-cadherin (yellow) and pFAK (purple) with and without ATC stimulation (30 min). (B) Corrected fluorescence intensity of E-cadherin, pFAK activation and Oct4 after 30 min ATC stimulation compared with control group. Scale bars 50 µm. All quantifications were from at least 3 independent experiments with two replicates per experiment. Unpaired t test p values *<0.05, **<0.01, and ***<0.001. n.s. not significant.