Figure 3
Localization of phosphorylated cyclic-AMP response element binding protein at Ser133 (pCREB (Ser133)) and 8-hydroxy-2′-deoxyguanosine (8-OHdG). (a) Immunohistochemical staining for pCREB, visualized by diaminobenzidine (brown) in cardiomyocyte (Arrowheads). (b) Quantification as the percentage of pCREB positively stained nuclei in total cardiomyocyte nuclei. (c) Immunohistochemical staining for 8-OHdG visualized by diaminobenzidine in cardiomyocyte (Arrowheads). (d) Quantification as the percentage of 8-OHdG positively stained nuclei in total cardiomyocyte nuclei. The box represents the 25th and 75th percentiles and the line the median value. Whiskers correspond to the 25th percentile minus 1.5 times interquartile range (IQR) and to the 75th percentile plus 1.5 IQR. *P < 0.05 vs. the dilated cardiomyopathy (DCM) group. †P < 0.05 vs. the normal control (NC) group. ‡P < 0.001 vs. the NC group. §P < 0.001 vs. the DCM group. TTS: takotsubo syndrome.
