Figure 2 | Scientific Reports

Figure 2

From: HDAC4 degradation by combined TRAIL and valproic acid treatment induces apoptotic cell death of TRAIL-resistant head and neck cancer cells

Figure 2

Combined TRAIL and VPA treatment induced caspase -dependent cell death signaling and decreased the expression of HDAC4. (A) HN3 cells were harvested, and FACS analysis was performed using anti-DR4 (left) or DR5 (right) antibody. Histograms display the fluorescence intensity (x-axis) versus relative cell numbers (y-axis). Black line, goat IgG isotype-control-labeled cells; red fill, cells only; green line, DR4-labeled cells; blue line, DR5-labeled cells. (B) HN3 cells were treated with TRAIL (0.5 µg/ml) and VPA (5 mM) for 24 h, and then Western blotting was performed with the following antibodies: DR4, DR5, Bax, XIAP, Bid, cleaved PARP, cleaved caspase 8, 7, 3, HDAC1, 2, 3, 4, 5, 7 and tubulin. (C) HN3 cells were pretreated with z-VAD for 1 h, followed by combined treatment TRAIL (0.5 µg/ml) and VPA (5 mM) for 24 h. Western blotting was performed with the following antibodies: HDAC4 and cleaved caspase 3.

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