Figure 10 | Scientific Reports

Figure 10

From: Isoform-specific hyperactivation of calpain-2 occurs presymptomatically at the synapse in Alzheimer’s disease mice and correlates with memory deficits in human subjects

Figure 10

Activity levels of calpain-2 are elevated in synaptosomes isolated from frontal neocortical tissue samples of post-mortem brains from AD patients. (A) Activity of synaptosomal calpain-2 immunoprecipitated from post-mortem brains of subjects with AD dementia and was found to be significantly higher compared to subjects with no cognitive impairment (NCI) or mild cognitive impairment (MCI). A significant negative correlation was observed between synaptosomal calpain-2 activity and performance of the subjects in tests for perceptual orientation (B) and speed (C), global cognition (D), semantic memory (E), episodic working (F) and as well as working memory (G) tests. Increase in calpain-2 activity in synaptosomal samples from post-mortem human brains also correlated with increase in amyloid deposits in the brain (H). Values are mean ± SD (n = 12 subjects) and * and # denote significant difference from NCI and MCI groups respectively (p < 0.05; ANOVA followed by post hoc tests with Newman-Keuls correction). See also Supplementary Fig. 2.

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