Figure 2
Aβ1–42 in cortical synaptosomes and in neurons derived from APP/PS1 mice. (A) Total Aβ1–42 levels in synaptosomes prepared from WT and APP/PS1 mice at 3 and 9 months of age were measured using ELISA. Data is represented as mean ± SD (n = 4 mice) and was analyzed using two-tailed Mann Whitney t-test. (B) The products formed following proteolysis of APP in the synaptosomes were detected by immunoblotting with 6E10 antibody and are represented as Aβ monomers, Aβ dimers/β-CTF and full length APP. Synthetic Aβ1–42 was used as standard to confirm the identity of the lower molecular weight bands. ‘*’ indicates a non-specific protein band detected in all samples. (C) Representative confocal images of primary neurons from WT and APP/PS1 mice, immunostained against homer1 (green) and Aβ42 (red) indicates co-localization of Aβ42 with homer1. Scale bar is 20 μm. (D) Representative overlay of high magnification confocal images of primary neurons from WT and APP/PS1 mice - Homer1 (green) and Aβ42 (red). Scale bar is 5 μm. (E) Quantification of Aβ42 signal from soma and neurites of both groups is shown. Data is represented as mean ± SD (n = 6–9 neurons and n = 16–36 neurites from each independent experiment). Mann Whitney two-tailed test was used for statistical analysis.
