Figure 1 | Scientific Reports

Figure 1

From: Characterization of membrane penetration and cytotoxicity of C9orf72-encoding arginine-rich dipeptides

Figure 1

Poly-PR localized to nucleoli. (A) Amino acid sequences of cell penetrating peptides (CPPs) used in this study. (B) Confocal imaging of live HEK293 cells treated with 10 μM of indicated FITC-labeled CPPs. Nucleus was visualized by Nucblue live dye. (C) Z-stack imaging of live HEK293 cells treated with FITC-(PR)20. (D) Immunocytochemistry of HEK293 cells treated with FITC-(PR)20, immunostained with anti-B23/nucleophosmin antibody. Nucleus was visualized by DAPI. (E) Hela cells were transfected with GFP-(PR)50 repeats. (F) Hela cells were transfected with GFP-(GR)50 repeats. White arrows show the cells with both nucleolar and cytosolic staining and white arrowheads indicate the cells with cytosolic staining. Scale bar shows 20 μm.

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