Figure 2

Poly-PR interacts with RNA. (A) Confocal imaging of HEK293 cells fixed/permeabilized and stained with FITC-(PR)20 in the absence of pretreatment of RNase A. (B) Colocalization analysis of signals from DAPI staining and FITC-(PR)20 in the HEK293 cells in the absence of pretreatment of RNase A. (C) Confocal imaging of HEK293 cells fixed/permeabilized and stained with FITC-(PR)20 in the presence of pretreatment of RNase A. (D) Colocalization analysis of signals from DAPI staining and FITC-(PR)20 in the HEK293 cells in the presence of pretreatment of RNase A. (E) Liquid-liquid phase separation of tetramethylrhodamine (TAMRA)-labeled random 15-mer RNA by FITC-(PR)20. (F) Agarose gel electrophoresis of human ribosomal RNA mixed with (PR)20 peptide at indicated concentration (μM). (G) Structure of RNA (base: adenine). (H) Crosslinking analysis of HA-(PR)20 peptide mixed with adenine, adenosine, ribose or ribose-phosphate in the presence or absence of RNA (poly-A). Arrowheads indicate crosslinked oligomer of HA-(PR)20 peptides. *polymerized HA-(PR)20.