Figure 3

Poly-PR caused cell death through inhibition of protein translation. (A) Scheme of real-time monitoring of protein translation using in vitro translation (IVT) system. tGFP: turboGFP. (B) Real time monitoring of tGFP signal in the IVT assay. Signals from tGFP were measured every 1 min. (C) Fluorescence intensity of translated tGFP in the IVT assay treated with 100 μM of each peptide. N = 3. (D) Cytotoxicity of each CPPs were monitored by release of LDH. NSC34 cells were treated with each CPP at indicated concentration for 24 hr. N = 6. (E) Cytotoxicity of each CPPs were monitored by Celltox glo luminescence. NSC34 cells were treated with each CPP at indicated concentration for 24 hr. N = 6. Each experiment was independently repeated at least 3 times. Asterisks indicate a significant difference analyzed by one-way ANOVA followed by Dunnett’s test (**p < 0.01, *p < 0.05).