Figure 5

Repeat lengths affected the cytotoxicity of poly-PR peptides. (A,B) Fluorescence intensity of translated tGFP in the IVT assay treated with 100 μM of each peptide. N = 3. (C) Cytotoxicity of poly-PR peptides with different repeat lengths were monitored by release of LDH. NSC34 cells were treated with each peptide at 5 μM or 10 μM for 24 hr. N = 6. (D) Confocal imaging of HEK293 cells stained with 10 μM of FITC-(PR)12 or FITC-(PR)20 before or after the fixation/permeabilization step. (E) CD spectra of R12, (PR)12 and (PR)20 in Tris-buffer (pH7.4). (F) CD spectra of R12, (PR)12 and (PR)20 in 1 v/w% SDS-Tris buffer (pH7.4). Asterisks indicate a significant difference analyzed by one-way ANOVA followed by Dunnett’s test (**p < 0.01, *p < 0.05).