Figure 6
Enforced delivery of poly-(PR)12 rescued the cytotoxicity. (A) Scheme of GE11 peptide-mediated endocytotic delivery system. (B) Cytotoxicity of GE11 peptide, (PR)12 peptide or GE11-(PR)12 peptides were monitored by release of LDH. Human fibroblasts were treated with each peptide at 10 μM, 20 μM or 50 μM for 48 hr. N = 6. (C) Microscopic image of human fibroblasts treated with 50 μM of each peptide for 48 hr. Each experiment was independently repeated at least 3 times. Asterisks indicate a significant difference analyzed by one-way ANOVA followed by Dunnett’s test (**p < 0.01, *p < 0.05).
